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Induction of TF and asTF mRNA by SubAB and Stx2. RNA was isolated from U937 cells or HUVECs treated with 1 μg/ml of SubAB or Stx2 for the indicated times. TF and asTF mRNA was measured using quantitative real time RT-PCR. Results are expressed as the fold change in [mRNA] relative to that in control (medium only) cells, and data shown are the mean ± SD for triplicate assays.
