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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Cell Res. 2010 Aug 3;20(10):1109–1116. doi: 10.1038/cr.2010.114

Figure 4. Ezh2-mediated H3K27 methylation represses lncRNA transcription in ES cells.

Figure 4

(A) Relative mRNA levels of Ezh2 in wildtype and Ezh2 knockdown ES cells as determined by RT-qPCR.

(B) Western blot analysis of H3K27me3 levels in Ezh2 KD ES cells.

(C) Box plot presentation of expression changes (KD1/ES) in all lncRNAs (n=24357) compared to lncRNAs marked by promoter H3K4me3 (n=6834) or H3K27me3 (n=1294) in ES cells. Significant p values were determined using the Student’s T test.

(D) Relative expression of lncRNAs upon Ezh2 knockdown in ES cells as determined by RT-qPCR. These lncRNAs were randomly selected from the list of lncRNAs that contain H3K27me3 in ES cells and are at least 2-fold upregulated upon Ezh2 knockdown as determined by microarray analysis.

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