Table 1.
E. coli strains used in this study.
| Strain | Relevant phenotype or genotype | Recipient | Source of allele | Reference |
|---|---|---|---|---|
| P90C | F− ara Δ(gpt-lac)5 thi | [54, 55] | ||
| AM887 | Δ(ruvA-ruvC)65 eda57::Cm | [31] | ||
| N3055 | uvrA277::Tn10 | Lloyd, R.G. | ||
| JC10289 | Δ(recA-srl)306 srlR301::Tn10 | [56] | ||
| A354 | recB21 argA::Tn10 sbcA | F.W. Stahl | ||
| WA576 | recF400::Tn5 | [57] | ||
| JJC2135 | recO1504::Tn5 | [58] | ||
| N2731 | recG258::Tn10Kn | [17] | ||
| N4452 | ΔrecG265::Cm | [59] | ||
| OH1000 | recQ::Cm | [60] | ||
| JW1172 | ΔumuD::Kn | [61] | ||
| YG7207 | ΔdinB::Kn | [62] | ||
| FC36 | F− ara Δ (gpt-lac)5 thi RifR (Pro−) | [55] | ||
| FC40 | FC36/ F’ Pro+ Φ(lacI33-lacZ) | [55] | ||
| FC348 | FC40 Δ(recA-srl)306 srlR301::Tn10 | [63] | ||
| FC404 | FC40 recB21 | [63] | ||
| FC438 | FC40 recG162 zib363::Tn10 | [63] | ||
| FC465 | FC40 recG258::Tn10Kn | [63] | ||
| FC485 | FC40 ruvA60::Tn10 | [63] | ||
| FC521 | FC40 recG258::Tn10Kn ruvA60::Tn10 | [63] | ||
| FC573 | FC40 Δ(ruvA-ruvC)65 | [63] | ||
| FC581 | FC40 Δ(ruvC)64::Kn | [63] | ||
| FC1354 | FC40 Δd i n B::Kn on chromosome and episome | [64] | ||
| FC1408 | FC40 ΔrecG265::Cm | [65] | ||
| FC1418 | FC40 lexA1 (Ind−) | [64] | ||
| PFB59 | F− parent of PFB60 | [66] | ||
| PFB60 | FC40 Δp o l B 1 | [66] | ||
| PFB208 | FC40 ΔpolB1 ΔdinB::Kn on chromosome and episome | PFB59 | YG7207 | This study |
| PFB236 | F− ara Δ (gpt-lac)5 thi RifR ΔdinB::Zeo | [65] | ||
| PFB241 | Met− donor of F’ Φ(lacI33-lacZ) ΔdinB::Zeo | This study | ||
| PFB243 | FC40 ΔdinB::Zeo on chromosome and episome | [65] | ||
| PFB286 | FC40 Δ(umuDC)::Erm | [67] | ||
| PFB287 | FC40 ΔpolB1 Δ(umuDC)::Erm | PFB286 | PFB60 | This study |
| PFB876 | FC40 ΔdinB::Kn Δ(umuDC)::Erm | PFB286 | YG7207 | This study |
| PFB878 | FC40 ΔdinB::Kn ΔpolB1 Δ(umuDC)::Erm | PFB876 | PFB60 | This study |
| PFB284 | FC36 Δ(umuDC)::Erm | This study | ||
| PFB873 | FC36 ΔdinB::Zeo Δ(umuDC)::Erm | This study | ||
| FC1536 | FC36 ΔumuD::Kn | FC36 | JW1172 | This study |
| PFB646 | FC40 ΔrecG265::Cm ΔdinB::Zeo on chromosome and episome | PFB243 | FC1408 | This study |
| PFB755 | FC40 uvrA277::Tn10 | FC40 | N3055 | This study |
| PFB756 | PFB243 uvrA277::Tn10 | PFB243 | N3055 | This study |
| PFB820 | FC40 lexA1 ΔdinB::Zeo on chromosome and episome | PF2015 | PFB243 | This study |
| PFB822 | FC40 ΔumuD::Kn | FC40 | JW1172 | This study |
| PFB881 | FC40 Δ(ruvA-ruvC)65ΔdinB::Zeo on chromosome and episome | PFB236 | AM887a | This study |
| PFB879 | FC40 recB21 ΔdinB::Zeo on chromosome and episome | PFB236 | A354b | This study |
| PFB827 | FC40 recF400 ΔdinB::Zeo on chromosome and episome | B243 | WA576 | This study |
| PFG404 | FC40 recF400::Tn5 | FC40 | WA576 | This study |
| PFG406 | FC40 recQ::Cm | FC40 | OH1000 | This study |
| PFG484 | FC40 recO1504::Tn5 | FC40 | JJC2135 | This study |
a
To introduce the Δ(ruvA-ruvC)65 allele, PFB236 was transduced to Cm resistance using a P1vir lysate grown on AM887 and transductants were screened for UV sensitivity. The episome from PFB241 was introduced by mating as described in the Materials and Methods (Section 2.1).
b
To introduce the recB21 allele, PFB236 was transduced to Tc resistance using a P1vir lysate grown on A354 and transductants were screened for UV sensitivity. The episome from PFB241 was introduced by mating as described in the Materials and Methods (Section 2.1).