Table 1.
PCR primers for BRAF, KRAS, and NRAS hotspot mutation analyses
Gene | Codon | Primer sequences (5′–3′) |
---|---|---|
KRAS | 12/13 | a 5′-GGCCTGCTGAAAATGACTGAA-3′ |
b 5′-GGTCCTGCACCAGTAATATGC-3′ | ||
KRAS | 61 | a 5′-CAGGATTCCTACAGGAAGCAAGTAG-3′ |
b 5′-CACAAAGAAAGCCCTCCCCA-3′ | ||
NRAS | 12/13 | a 5′-ATGACTGAGTACAAACTGGT-3′ |
b 5′-CTCTATGGTGGGATCATATT-3′ | ||
NRAS | 61 | a 5′-TCTTACAGAAAACAAGTGGT-3′ |
b 5′-AGCGGATAACAATTTCACACAGGC CAA AAA TTTAATCAGTGGA-3′ | ||
BRAF | 600 | a 5′-TGTAAAACGACGGCCAGTCATAATGCTTGCTCTGA TAG GA-3′ |
b 5′-AGCGGATAACAATTTCACACAGGCCAA AAATTTAATCAGTGGA-3′ |
a Forward, b reverse