TABLE 1.

Viral shedding during acute infection and after Dx administration and presence of viral antigens in brain sections of calves experimentally inoculated with a BHV-5 isolate^a

Group and animal	Virus shedding:		VN titer(s)		Presence ofc:
	During Acute-infection (days p.i.)	After Dx (day[s] p.r.)a	30 days p.i.	30 days p.r.b	Neurological disease	Viral antigenf
Group A
101	3-20	NR	32	NT	−	−
103	3-21	NR	16	NT	−	−
111	3-21	NR	32	NT	−	−
116	2-21	NR	16	NT	−	−
Group B
109	3-20	8-13, 15	64	64	−	−
112	3-21		16	64	−	−
117	3-21	10	16	64	−	−
122	3-20	16	16	16	−	−
124	3-19	4, 5, 8-16	64	256	−	−
118	3-20	7, 8	16	NT	+ (8c)	+
121	3-21		16	NT	+ (7e)	+
123	2-21		32	NT	+ (8e)	+
Controls
C1			<2	NT	−	−
C2			<2	<2	−	−

^aNR, Not submitted to reactivation.

^bNT, not tested.

^c+, positive for neurological disease or viral antigens; −, negative for neurological disease or viral antigens.

^dDay of onset of neurological signs.

^eDay died or were sacrificed in extremis.

^fIHC was performed during latent infection in the brains of calves from group A (55 days p.i.) and B (55 days, p.r.) to demonstrate the absence of viral replication or gene expression. In calves 118, 121, and 123, IHC was performed to demonstrate viral replication or gene expression associated with the neurological disease.