Table 3.

Some molecular and biochemical properties of purified Phy I and Phy II from Aspergillus niger NCIM 563

Properties	Values
	Phy I	Phy II
pH
Optimum	2.5	5.0
Stability	2.0–9.0	4.0–8.5
Temperature
Optimum	55°C	55°C
Stability	t 1/2 at 55°C, 6–8 h	t 1/2 at 55°C, 10–12 h
Isoelectric point	3.65	3.95
Molecular mass (kDa)
Native	264	150–160
Subunit (SDS)	66	150
MALDI	66	74
Glycoprotein nature	Glycosylated	Non-glycosylated
Substrate specificity	Broad	Specific for Na phytate