TABLE 2.
Fragment no. | RAPD primer | Fragment size (nt) | Sequence length (nt) | Sequence homol- ogy | Forward primer
|
Reverse primer
|
Annealing temp (°C) | ||
---|---|---|---|---|---|---|---|---|---|
No. | Sequence | No. | Sequence | ||||||
1 | 695 | 600 | 585 | None | 989 | AGAAAGCGGACGGAGAGCAAAATG | 990 | TATGACAAACACAGGGACAACGAG | 54 |
5 | 701 | 1,550 | 1,467 | None | 1144 | AGGGTGAATTTGAGCCAGTTGAGT | 1145 | AGTGGGGCGAGTACAGGGATAATA | 55 |
7 | 701 | 1,000 | 811 | None | 995 | TGCTGCTGCTGCTCCTCCTGTTAC | 996 | CGACTACCACCACCGCCACTACT | 59 |
8 | 701 | 800 | 682 | None | 998 | AGTTTGGCACGAGGAAGGTCATTG | 999 | TTTGGCGAAGTTTGGGTAGGATGG | 54 |
15 | 52 | 1,050 | 1,061 | cumA | 813 | TTTCAGGCTCAGGGTGCGTTCGTG | 814 | GTTTAGGTGATGCCGGTGTATGGT | 56 |
16 | 52 | 800 | 793 | None | 810 | CGGTCGGCGGTTAGAGAAAT | 811 | TCGGGGGCCACTGCTGCTA | 50 |
17 | 46 | 450 | 439 | None | 1192 | TGCACGTGGAATCGCATAATAATA | 1193 | GAGAATTGCACGGAAGTTTGGTAA | 55 |