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. 2003 Oct;41(10):4537–4541. doi: 10.1128/JCM.41.10.4537-4541.2003

TABLE 2.

RAPD fragments cloned and used for PCR-RFLP procedure

Fragment no. RAPD primer Fragment size (nt) Sequence length (nt) Sequence homol- ogy Forward primer
Reverse primer
Annealing temp (°C)
No. Sequence No. Sequence
1 695 600 585 None 989 AGAAAGCGGACGGAGAGCAAAATG 990 TATGACAAACACAGGGACAACGAG 54
5 701 1,550 1,467 None 1144 AGGGTGAATTTGAGCCAGTTGAGT 1145 AGTGGGGCGAGTACAGGGATAATA 55
7 701 1,000 811 None 995 TGCTGCTGCTGCTCCTCCTGTTAC 996 CGACTACCACCACCGCCACTACT 59
8 701 800 682 None 998 AGTTTGGCACGAGGAAGGTCATTG 999 TTTGGCGAAGTTTGGGTAGGATGG 54
15 52 1,050 1,061 cumA 813 TTTCAGGCTCAGGGTGCGTTCGTG 814 GTTTAGGTGATGCCGGTGTATGGT 56
16 52 800 793 None 810 CGGTCGGCGGTTAGAGAAAT 811 TCGGGGGCCACTGCTGCTA 50
17 46 450 439 None 1192 TGCACGTGGAATCGCATAATAATA 1193 GAGAATTGCACGGAAGTTTGGTAA 55