Table 3.
Transgenic lines used for the analysis of tectum structure or function in zebrafish
| Short name | Full name | Description | References |
|---|---|---|---|
| Pou4f3:mGFP (Brn3c:mGFP), Pou4f3:Gal4 (Brn3c:Gal4) | Tg(pou4f3:gap43-gfp)s356t, Tg(pou4f3:gap43-gfp)s273t, Tg(pou4f3:gal4-vp16)s311t | Labels a subset (40%) of RGCs; projection into SO, SFGSD and SFGSF | [10,12,22,95] |
| BGUG | Tg(pou4f3:gal4-vp16, UAS:gap43-gfp)s314t, Tg(pou4f3:gal4-vp16, UAS:gap43-gfp)s318t | Labels a random subset of Pou4f3-positive RGCs with membrane-bound GFP; also drives GFP expression in random cells within any Gal4 pattern ('genetic Golgi') | [12,20,21] |
|
Ath5:GFP (Atoh7:GFP), Ath5:mGFP, Ath5:mRFP, Ath5:GCaMP1.6, Ath5:Gal4 |
Tg(atoh7:gfp), Tg(Atoh7:gap43-GFP)cu1, Tg(Atoh7:gap43-RFP)cu2, Tg(atoh7:gcamp1.6), Tg(atoh7:gal4-vp16) | Labels 100% of RGCs and some retinal interneurons | [42,96-98] |
| Isl2b:GFP, Isl2b:mCherry-CAAX, Isl2b:mGFP | Tg(-17.6isl2b:GFP)zc7, Tg(-17.6isl2b:mCherry-HsHRAS)zc23, Tg(-17.6isl2b:gap43-GFP)zc20, | Labels all or the vast majority of RGCs | [99] |
| Pou4f1: GFP (Brn3a:GFP), Pou4f1:Gal4 (Brn3a:Gal4) | Tg(pou4f1-hsp70l:gfp)rw0110b | Labels RGCs. Also labels many PVNs, including glutamatergic PVPNs with ipsilateral axons to the hindbrain and GABAergic neurons with tectotectal axons | [27] |
| Gal4s1013t | Et(-1.5hsp70l:gal4-vp16)s1013t | Drives expression in all neurons and glia of the tectum | [20] |
| Gal4s1038t | Et(fos:gal4-vp16)s1038t | Drives expression in PVPNs of the posterior tectum | [20] |
| Gal4s1156t | Et(-1.5hsp70l:gal4-vp16)s1156t | Drives expression in very few tectal neurons, including most SINs | [54] |
| Gal4s1101t | Et(e1b:gal4-vp16)s1101t | Drives expression in almost all neurons of the CNS; 'pan-neural' | [55,59,60] |
CNS, central nervous system.