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. 2010 Jul 13;12(4):R48. doi: 10.1186/bcr2605

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Copyright ©2010 Ghebeh et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The effect of doxorubicin on B7-H1 cellular distribution. A) Representative confocal immunofluorescence image of MDA-MB-231 cells monolayer after drug treatments. The overlay of B7-H1 (green) DAPI (nucleus blue) is shown as bluish white color. Numbers beside drugs indicates the concentration in μg/mL. B and D) Western blot showing B7-H1 and phospho-AKT expression respectively in different protein fractions of MDA-MB-231 cells treated with doxorubicin for 72 hours. C) Western blot showing B7-H1 expression in different protein fractions of T47 D cells treated with doxorubicin for 72 hours. GAPDH was used as control as well as for quantification of the B7-H1 expression.