Table 2.
Metabolic enzymes identified in AsPC-1 cells
| Protein name | Accession # | Unique peptides | Total peptides | Mr (Kda) | PI | Biological process |
|---|---|---|---|---|---|---|
| 2-oxoglutarate dehydrogenase E1 component, mitochondrial precursor | ODO1_HUMAN | 8 | 18 | 115.9 | 6.39 | Glycolysis |
| 3,2-trans-enoyl-CoA isomerase, mitochondrial precursor | D3D2_HUMAN | 3 | 13 | 32.8 | 8.8 | Fatty acid metabolism; Lipid metabolism |
| 3-hydroxyacyl-CoA dehydrogenase type-2 | HCD2_HUMAN | 6 | 10 | 26.9 | 7.65 | Lipid metabolic process; tRNA processing |
| 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor | 3HIDH_HUMAN | 7 | 16 | 35.3 | 8.38 | Pentose-phosphate shunt; valine metabolic process |
| 3-ketoacyl-CoA thiolase, peroxisomal precursor | THIK_HUMAN | 3 | 4 | 44.3 | 8.76 | Fatty acid metabolism; Lipid metabolism |
| 3-mercaptopyruvate sulfurtransferase | THTM_HUMAN | 3 | 7 | 33.2 | 6.13 | Cyanate catabolic process |
| 78 kDa glucose-regulated protein | GRP78_HUMAN | 7 | 12 | 72.3 | 5.07 | ER-associated protein catabolic process; ER unfolded protein response; ER regulation of protein folding |
| Acetyl-CoA acetyltransferase, mitochondrial precursor | THIL_HUMAN | 2 | 6 | 45.2 | 8.98 | Ketone body metabolism |
| Aconitate hydratase, mitochondrial | ACON_HUMAN | 2 | 3 | 85.4 | 7.36 | Tricarboxylic acid cycle |
| Acyl-protein thioesterase 1 | LYPA1_HUMAN | 2 | 2 | 24.7 | 6.29 | Fatty acid metabolism; Lipid metabolism |
| Adenylate kinase 2, mitochondrial | KAD2_HUMAN | 7 | 20 | 26.5 | 7.67 | Nucleic acid metabolic process |
| ADP/ATP translocase 2 | ADT2_HUMAN | 5 | 11 | 32.9 | 9.76 | Transmembrane transporter activity |
| Aldehyde dehydrogenase, mitochondrial | ALDH2_HUMAN | 3 | 7 | 56.3 | 6.63 | Alcohol metabolic process |
| Alpha-enolase | ENOA_HUMAN | 2 | 2 | 47.1 | 7.01 | Glycolysis |
| Amine oxidase B | AOFB_HUMAN | 2 | 2 | 58.7 | 7.2 | Oxidation reduction |
| Aspartate aminotransferase, mitochondrial | AATM_HUMAN | 4 | 6 | 47.4 | 9.14 | Lipid transport |
| ATP synthase subunit alpha, mitochondrial | ATPA_HUMAN | 21 | 52 | 59.7 | 9.16 | ATP synthesis |
| ATP synthase subunit d, mitochondrial | ATP5H_HUMAN | 3 | 7 | 18.5 | 5.21 | ATP synthesis; Ion transport |
| ATP synthase subunit b, mitochondrial | AT5F1_HUMAN | 2 | 3 | 28.9 | 9.37 | ATP synthesis |
| ATP synthase subunit beta, mitochondrial | ATPB_HUMAN | 28 | 95 | 56.5 | 5.26 | ATP synthesis |
| ATP synthase subunit f, mitochondrial | ATPK_HUMAN | 2 | 2 | 10.9 | 9.7 | ATP synthesis; Ion transport |
| ATP synthase subunit gamma, mitochondrial; | ATPG_HUMAN | 3 | 6 | 33 | 9.23 | ATP synthesis; proton transport |
| ATP synthase subunit O, mitochondrial | ATPO_HUMAN | 6 | 11 | 23.3 | 9.97 | ATP synthesis, ion transport; ATP catabolic process |
| Calcium-binding mitochondrial carrier protein Aralar2 | CMC2_HUMAN | 7 | 16 | 74.1 | 7.14 | Mitochondrial aspartate and glutamate carrier |
| Citrate synthase, mitochondrial precursor | CISY_HUMAN | 2 | 3 | 51.7 | 8.45 | Tricarboxylic acid cycle |
| Cytochrome b5 type B | CYB5B_HUMAN | 2 | 4 | 16.3 | 4.88 | Electron transport |
| Cytochrome b-c1 complex subunit 1, mitochondrial | QCR1_HUMAN | 6 | 12 | 52.6 | 5.94 | Electron transport |
| Cytochrome b-c1 complex subunit 2, mitochondrial | QCR2_HUMAN | 3 | 4 | 48.4 | 8.74 | Aerobic respiration; electron transport chain; oxidative phosphorylation |
| Cytochrome c oxidase subunit 2 | COX2_HUMAN | 2 | 6 | 25.5 | 4.67 | Electron transport chain |
| Cytochrome c1, heme protein, mitochondrial | CY1_HUMAN | 5 | 10 | 35.4 | 9.15 | Electron transport chain |
| Cytochrome c1, heme protein, mitochondrial | CY1_HUMAN | 2 | 3 | 35.4 | 9.15 | Electron transport chain |
| D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor | BDH_HUMAN | 2 | 3 | 38.1 | 9.1 | Oxidation reduction |
| Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial | ECH1_HUMAN | 4 | 10 | 35.8 | 8.16 | Fatty acid metabolism; Lipid metabolism |
| Delta-1-pyrroline-5-carboxylate synthetase | P5CS_HUMAN | 2 | 4 | 87.2 | 6.66 | Amino-acid biosynthesis; Proline biosynthesis |
| Dihydrolipoyl dehydrogenase, mitochondrial | DLDH_HUMAN | 7 | 16 | 54.1 | 7.95 | Cell redox homeostasis |
| Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial | ODP2_HUMAN | 3 | 5 | 65.7 | 7.96 | Glycolysis |
| Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial | ODO2_HUMAN | 4 | 7 | 48.6 | 9.01 | Tricarboxylic acid cycle |
| Electron transfer flavoprotein subunit alpha, mitochondrial | ETFA_HUMAN | 2 | 5 | 35.1 | 8.62 | Electron transport |
| Electron transfer flavoprotein subunit beta | ETFB_HUMAN | 4 | 6 | 27.8 | 8.25 | Electron transport |
| Endoplasmin | ENPL_HUMAN | 16 | 28 | 92.4 | 4.76 | ER-associated protein catabolic process; protein folding/transport; response to hypoxia |
| Enoyl-CoA hydratase, mitochondrial | ECHM_HUMAN | 9 | 26 | 31.4 | 8.34 | Fatty acid metabolism; Lipid metabolism |
| Glutamate dehydrogenase 1, mitochondrial; | DHE3_HUMAN | 3 | 4 | 61.4 | 7.66 | Glutamate metabolism |
| Glyceraldehyde-3-phosphate dehydrogenase | G3P_HUMAN | 5 | 7 | 36 | 8.57 | Glycolysis |
| Glycerol-3-phosphate dehydrogenase, mitochondrial precursor | GPDM_HUMAN | 8 | 15 | 80.8 | 7.23 | Glycolysis |
| Haloacid dehalogenase-like hydrolase domain-containing protein 3 | HDHD3_HUMAN | 3 | 4 | 28 | 6.21 | Metabolic process phosphoglycolate phosphatase activity |
| Histidine triad nucleotide-binding protein 2 | HINT2_HUMAN | 2 | 3 | 17.2 | 9.2 | Lipid synthesis; Steroid biosynthesis |
| Hyaluronidase-3 | HYAL3_HUMAN | 2 | 2 | 46.5 | Carbohydrate metabolic process | |
| Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial precursor | HCDH_HUMAN | 2 | 4 | 34.3 | 8.88 | Fatty acid metabolism; Lipid metabolism |
| Isoleucyl-tRNA synthetase, mitochondrial precursor | SYIM_HUMAN | 5 | 7 | 113.7 | 6.78 | Protein biosynthesis |
| Isovaleryl-CoA dehydrogenase, mitochondrial | IVD_HUMAN| | 2 | 2 | 46.3 | 8.45 | Leucine catabolic process; Oxidation reduction |
| L-lactate dehydrogenase A chain | LDHA_HUMAN | 3 | 5 | 36.7 | 8.84 | Glycolysis |
| Lon protease homolog, mitochondrial | LONM_HUMAN | 2 | 2 | 106.4 | 6.01 | Required for intramitochondrial proteolysis |
| Long-chain-fatty-acid--CoA ligase 5; | ACSL5_HUMAN | 2 | 4 | 75.9 | 6.49 | Fatty acid metabolism; Lipid metabolism |
| Malate dehydrogenase, mitochondrial | MDHM_HUMAN | 3 | 5 | 35.5 | 8.92 | Tricarboxylic acid cycle; Glycolysis |
| Medium-chain specific acyl-CoA dehydrogenase, mitochondrial | ACADM_HUMAN | 2 | 6 | 46.6 | 8.61 | Fatty acid metabolism; Lipid metabolism |
| Mitochondrial carrier homolog 2 | MTCH2_HUMAN | 3 | 10 | 33.3 | 8.25 | Transmembrane transport |
| Mitochondrial inner membrane protein | IMMT_HUMAN | 2 | 2 | 83.6 | 6.08 | Protein binding; Cell proliferation-inducing |
| NADH-cytochrome b5 reductase 3 | NB5R3_HUMAN | 3 | 3 | 34.2 | 7.18 | Cholesterol biosynthesis; Lipid/steroid synthesis |
| Neutral alpha-glucosidase AB | GANAB_HUMAN | 6 | 9 | 106.8 | 5.74 | Carbohydrate metabolic process |
| Peptidyl-prolyl cis-trans isomerase A | PPIA_HUMAN | 2 | 3 | 18 | 7.68 | Protein folidng; Interspecies interation |
| Peroxiredoxin-5 | PRDX5_HUMAN | 2 | 5 | 22 | 8.85 | Cell redox homeostasis |
| Phosphoenolpyruvate carboxykinase, mitochondrial | PPCKM_HUMAN | 8 | 18 | 70.6 | 7.56 | Gluconeogenesis |
| Phosphoglycerate kinase 1 | PGK1_HUMAN | 4 | 7 | 44.6 | 8.3 | Glycolysis |
| Protein disulfide-isomerase | PDIA1_HUMAN | 3 | 3 | 57.1 | 4.76 | Cell redox homeostasis |
| Protein disulfide-isomerase A3 | PDIA3_HUMAN | 4 | 7 | 56.7 | 5.98 | Cell redox homeostasis |
| Protein disulfide-isomerase A4 | PDIA4_HUMAN | 2 | 2 | 72.9 | 4.96 | Cell redox homeostasis; Protein secretion |
| Protein disulfide-isomerase A6 | PDIA6_HUMAN | 2 | 3 | 48.1 | 4.95 | Cell redox homeostasis; Protein folding |
| Protein ETHE1, mitochondrial | ETHE1_HUMAN | 4 | 11 | 27.9 | 6.35 | Metabolic homeostasis in mitochondria |
| Protein transport protein Sec16A | SC16A_HUMAN | 2 | 2 | 233.4 | 5.4 | ER-Golgi transport; Protein transport |
| Pyruvate dehydrogenase E1 component alpha subunit, somatic form | ODPA_HUMAN | 2 | 4 | 43.3 | 8.35 | Glycolysis |
| Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial precursor | ODPAT_HUMAN | 3 | 7 | 42.9 | 8.76 | Glycolysis |
| Pyruvate dehydrogenase E1 component subunit beta, mitochondrial | ODPB_HUMAN | 2 | 3 | 39.2 | 6.2 | Glycolysis; Tricarboxylic acid cycle |
| Serine hydroxymethyltransferase, mitochondrial | GLYM_HUMAN | 12 | 21 | 56 | 8.76 | L-serine metabolic process; Glycine metabolic process; One-carbon metabolic process |
| Succinate dehydrogenase flavoprotein subunit, mitochondrial | DHSA_HUMAN | 2 | 5 | 72.6 | 7.06 | Electron transport; Tricarboxylic acid cycle |
| Succinyl-CoA ligase [GDP-forming] beta-chain, mitochondrial precursor | SUCB2_HUMAN | 3 | 3 | 46.5 | 6.15 | Succinyl-CoA metabolic process; Tricarboxylic acid cycle |
| Succinyl-CoA ligase [GDP-forming] subunit alpha, mitochondrial precursor | SUCA_HUMAN | 2 | 5 | 35 | 9.01 | Tricarboxylic acid cycle |
| Superoxide dismutase [Mn], mitochondrial | SODM_HUMAN | 2 | 5 | 24.7 | 8.35 | Elimination of radicals |
| Thioredoxin-dependent peroxide reductase | PRDX3_HUMAN | 4 | 10 | 27.7 | 7.68 | Cell redox homeostasis; Hydrogen peroxide catabolic process |
| Thiosulfate sulfurtransferase | THTR_HUMAN | 2 | 3 | 33.4 | 6.77 | Cyanate catabolic process |
| Trifunctional enzyme subunit alpha, mitochondrial | ECHA_HUMAN | 17 | 46 | 82.9 | 9.16 | Fatty acid metabolism; Lipid metabolism |
| Trifunctional enzyme subunit beta, mitochondrial | ECHB_HUMAN | 6 | 12 | 51.3 | 9.45 | Fatty acid metabolism |
| Trimethyllysine dioxygenase, mitochondrial | TMLH_HUMAN | 2 | 3 | 49.5 | 7.64 | Carnitine biosynthesis |
| Very long-chain specific acyl-CoA dehydrogenase, mitochondrial | ACADV_HUMAN | 3 | 5 | 70.3 | 8.92 | Fatty acid metabolism; Lipid metabolism |