Figure 1.

Schema for OVA sensitization and challenge of mice, isolation of a total lung fibroblast fraction, and sorting of a mesenchymal stromal cell fraction. Mice were sensitized twice by intraperitoneal (IP) injections at day 0 and day 11 of OVA followed by intranasal (IN) challenges at days 11, 18, 21, 22, and 23. Lungs were isolated, digested with collagenase, and adherent cells plated with daily media changes to remove nonadherent cells. At the end of 5 days, adherent cells were lifted, sorted and, in two additional weeks, processed for colony formation.