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. 2010 Sep 10;5:23. doi: 10.1186/1747-1028-5-23

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Copyright ©2010 Robbins and Cross; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Overexpressed CDH1-m11 lowers Cdc20 levels, but does not clear it. Either HA-CDH1 or HA-CDH1-m11 was induced using deoxycorticosterone in cycling strains with a GAL4-Mineralocorticoid receptor fusion (containing the DNA binding domain of the former, and ligand binding domain of the latter, rendering GAL responsive genes inducible by exogenous mineralocorticoids [38]). Levels of endogenously expressed 18MYC-Cdc20 and HA-Cdh1 were followed by immunoblot, with a nonspecific band reactive with the anti-HA antibody serving as a loading control.