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. 2010 Sep 17;10:273. doi: 10.1186/1471-2334-10-273

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Copyright ©2010 Chen et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Purification and analysis of recombinant Rv1985c. (A) Electrophoresis analysis on a 12% SDS-PAGE gel stained with Coomassie blue; Lane 1, Takara low molecular weight marker; Lane 2, crude E. coli supernatant fraction before application to column (induced with IPTG); Lane 3, flow-through fraction of His-bind resin; Lane 4, fraction washed with 120 mM imidazole; Lane 5, fraction eluted with 250 mM imidazole. Molecular weight of Rv1985c is approximately 33.8 kDa and protein marker sizes are indicated on the side of gel. (B) Mass-fingerprinting protein assay of 33.9-kDa by MALDITOF.