Figure 2.
ELISPOT showed that PBMC from both TB and LTBI groups developed stronger IFN-γ producing response to Rv1985c antigens than those from HC (healthy controls) group. PBMC from TB, LTBI, and HC individuals were stimulated overnight with recombinant Rv1985c and IFN-γ producing T cells were detected by ELISPOT. Results of individual response are expressed as spot forming units (SFU) per 2.5 × 105 PBMCs. Horizontal lines indicate the median of SFU of each group. SFU greater than or equal to 6 was considered positive (indicated by the dotted line). P-values of SFU difference between either two groups were shown above the plots determined by Kruskale-Wallis one-way analysis Dunn's post-test. ***, P < 0.001; **, P < 0.01; *, P < 0.05; NS: P-value is not significant.