Table 2.
Characterization of IFN-γ ELISPOT in response to recombinant Rv1985c and the peptides mixture from ESAT-6 and CFP-10 in TB patients, LTBI and Healthy controls groups:
| TB (n = 56) | LTBI (n = 20) | HC (n = 30) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Antigens |
Mean SFU ± standard error |
No. positive1 | Sensitivity2 (%) | 95% CI (%) |
Mean SFU ± standard error |
No. positive1 | Sensitivity3 (%) | 95% CI (%) |
Mean SFU ± standard error |
No. positive1 | Specificity4 (%) | 95% CI (%) |
| Rv1985c | 19.3 ± 4.7 | 40 | 71.4 | 57.8 to 82.7 | 10.0 ± 2.8 | 11 | 55 | 31.1 to 78.9 | 2.6 ± 1.1 | 1 | 96.7 | 82.8 to 99.9 |
| ESAT-6 | 66.59 ± 9.35 | 46 | 82.16 | 70.6 to 91.2 | 37.6 ± 12.0 | 15 | 75 | 54.2 to 95.8 | 0.77 ± 0.20 | 0 | 100.0 | 88.4 to 100.0 |
| CFP-10 | 132.8 ± 22.25 | 38 | 67.97 | 54.9 to 80.2 | 77.9 ± 24.0 | 15 | 75 | 54.2 to 95.8 | 1.0 ± 0.25 | 0 | 100.0 | 88.4 to 100.0 |
1: Positive was defined as SFU greater than or equal to 6 in 2.5 × 105 cells for ESAT-6 and CFP-10, and greater than 4.5 in 2.5 × 105 cells for Rv1985c;
2: Percentage of responding patients out of all TB patients tested;
3: Percentage of responding individuals out of all LTBI tested;
4: Percentage of true-negative healthy controls out of all healthy control individuals tested;
5: Statistically higher SFU than SFU of Rv1985c by two-tailed paired Mann-Whitney test, p < 0.001;
6: Statistical significance was determined in respect to sensitivity of Rv1985c by McNemar chi-square test, p = 0.02;
7: Not significant in respect to sensitivity of Rv1985c by McNemar chi-square test, p = 0.52.