Figure 1.
Effect of 10% FBS, delipidated FBS, and human serum on the ability of Mip to induce TNF-α and IL-8 production by PMA-differentiated THP-1 cells. Cells (2.5 × 105 cells/1 ml per well) were cultured with 10 nM PMA for 48 h and then stimulated by recombinant Mip (1 μg/ml) in the presence or absence of 10% heat-inactivated serum. After 4 h stimulation, supernatants were collected and their content in TNF-α and IL-8 were analyzed by ELISA. Each value represents the mean ± SD of triplicates from four experiments. ***: p < 0.0001 determined by comparison with medium alone using Student's t test.