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. 2010 Sep 19;10:69. doi: 10.1186/1472-6750-10-69

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Copyright ©2010 Seo et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of eGFP expression driven by CMV or CAG promoter in DF-1 cells. The effect of various cis-acting elements on eGFP expression were analyzed in both mRNA (a) and protein (b) levels. A significant model effect was found on both mRNA and MFI (both P < 0.0001). Means with different superscripts (a, b, c, d, e, f) differ significantly (P < 0.05; F values = 15.80 and 67.51 in mRNA and protein level analysis, respectively; total degree of freedom = 23). Data represent the mean of three replicates, and error bars indicate ± S.D. (n = 3 in each plasmid).