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. 2010 Sep 13;9:239. doi: 10.1186/1476-4598-9-239

Figure 4.

Figure 4

WithaD activates MKK group of kinases upstream of p38 and JNK. (A) K562 and MOLT-4 cells were treated with WithaD (1.5 and 0.5 μM respectively) for different time points. The level of phosphorylation of MKK4, MKK7 and MKK3/6 was determined in WithaD-treated cells by Western blot analysis using appropriate anti-MKK antibody. β-actin served as loading control. (B) The level of phosphorylation of MKK4, MKK7 and MKK3/6 was further determined after 2 hr treatment with different amount (0-4 μM) of WithaD as described above.