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. 2010 Sep 16;9:246. doi: 10.1186/1476-4598-9-246

Figure 3.

Figure 3

HOX11 is Phosphorylated on Threonine-247. (A) Immunoblots of anti-HOX11 immunoprecipitates using anti-HOX11, anti-pThr and anti-pSer antibodies. (B) Schematic diagram of expression constructs for FLAG-tagged HOX11 wildtype and mutant proteins. FLAG peptide, PBX1-interacting domain (PIM domain), glutamine-rich stretch (QQxQQ) and homeodomain (HD) are noted. (C) Top Panel: HOX11 immunoblots of wildtype FLAG-HOX11 and truncation mutants with and without nocodazole treatment. Middle Panel: Autoradiogram of in vitro 35S-γATP-labelled FLAG-HOX11 species. NIH 3T3 cells transfected with wildtype FLAG-HOX11 or truncation mutations were pulsed with 35S-γATP either with or without nocodazole. Anti-FLAG immunoprecipitates were obtained as described in the Materials and Methods. Bottom Panel: β-actin immunoblot of wildtype FLAG-HOX11 and truncation mutants with and without nocodazole treatment. (D) Top Panel: HOX11 immunoblots of wildtype FLAG-HOX11, T247A and T241A point mutants with and without nocodazole treatment. Middle Panel: Autoradiogram of in vitro 35S-γATP-labelled FLAG-HOX11 species. Bottom Panel: β-actin immunoblot of wildtype FLAG-HOX11 and point mutants with and without nocodazole treatment.