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. 2010 Sep 22;9:255. doi: 10.1186/1476-4598-9-255

Figure 4.

Figure 4

Function of myosin II-B in BLM melanoma cell migration and invasion. (A) Western blot to verify silencing of myosin II-B expression 72 h post-transfection in BLM LIE and BLM P-cad cells. Matrigel invasion assay shows reduced invasion of BLM LIE siMyosin II-B when compared to control cells (p < 0,0001). No significant difference could be observed for BLM P-cad siMyosin II-B compared to BLM P-cad siControl (p = 0,2). (B) Reduced migration of BLM LIE siMyosin II-B compared to BLM LIE siControl could be observed (p = 0,008) (upper panel). No significant difference between BLM P-cad siControl and siMyosin II-B could be detected (p = 0,7). Results of a wound healing assay with addition of specific kinase inhibitors (lower panel). KT-5926 significantly reduced cellular migration when compared to control situation, in contrast to Y27632 (p = 0,009; p = 0,98).