Table 1.
Comparison between the main fatty acid composition of freshly obtained human lymphocytes and lymphoblastoid cell lines transfected with the empty vector.
| Fresh | EBV immortalized | |
|---|---|---|
| 14:0 | 0.49 | 2.06 |
| 16:0 | 19.59 | 21.06 |
| 16:1ω9 | 0.28 | 2.21 |
| 16:1ω7 | 0.28 | 1.27 |
| 18:0 | 19.72 | 15.66 |
| 18:1ω9 | 15.30 | 19.29 |
| 18:1ω7 | 1.68 | 7.29 |
| 18:2ω6 | 7.49 | 2.75 |
| 18:3ω6 | 0.03 | 0.09 |
| 18:3ω3 | 0.09 | 0.06 |
| 20:0 | 1.00 | 0.25 |
| 20:1ω9 | 0.64 | 1.45 |
| 20:3ω9 | 0.10 | 0.74 |
| 20:3ω6 | 1.65 | 3.21 |
| 20:4ω6 | 19.79 | 7.06 |
| 20:5ω3 | 0.31 | 0.58 |
| 22:4ω6 | 2.13 | 1.23 |
| 22:5ω6 | 0.37 | 0.31 |
| 22:5ω3 | 1.34 | 4.20 |
| 22:6ω3 | 2.01 | 4.46 |
| ω6/ω3 | 7.72 | 1.41 |
| 22:6ω3/22:5ω3 | 1.50 | 1.06 |
| 22:5ω6/22:4ω6 | 0.17 | 0.26 |
| 20:4ω6/20:3ω6 | 11.99 | 2.20 |
| 22:4ω6/20:4ω6 | 0.11 | 0.17 |
This table compares the main fatty acid composition (mol%) of freshly processed human lymphocytes with that of lymphoblastoid cell lines immortalized with the Epstein-Barr virus and transfected with the empty plasmid pIRES2EGFP (mean). Note the very different arachidonic acid content of fresh and treated cells. In contrast, the ω3 PUFAs are higher and the 22:6ω3/22:5ω3 ratio is comparatively lower in the processed cells. Also, note the higher values of ω9 and ω7 fatty acids in the EBV-treated cells.