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. 2010 Sep 13;10:276. doi: 10.1186/1471-2148-10-276

Table 6.

Description of PCR programs.

Program Description
Std-Fix 3' 94°; 35 × [40" 94°C; 1' (45, 48, 50, or 55°C according to experiment), 2'72°C], 3'72°C
Fix50-60°C 3' 94°; 8 × [30" 94°C; 1' 50°C, 1'72°], 26 × [30" 94°C; 30"60°C; 1'72°C], 3'72°C
TD6 2'94°C; 14 × [1'94°C,1'58°C to 45°C (- 1°C/cycle), 1'72°C], 25 × [30"94°C, 45"58°C, 45"72°C], 3'72°C
TD6' 2'94°C; 14 × [1'94°C,1'58°Cto 45°C (- 1°C/cycle), 1'72°C], 25 × [40"94°C, 40"58°C, 1'72°C], 3'72°C
EE-GP 2' 94°; 35 × [20" 94°C; 1' 45°C; 1'72°]; 5' 72°C
DA 3' 94°C; 30 × [1' 94°C; 1' 45°C; 1'72°C]; 5' 72°C

DA, EE and GP refer to initials of the authors who used them. ': minute. ": second.