Figure 3. ATP is required for DHX9 helicase activity.
Panel A. Triplex DNA (see legend to Figure 1) was incubated with 0–200 nM DHX9 helicase in the presence (left) or in the absence (right) of 5 mM ATP. Panel B. Triplex DNA was incubated with 200 nM DHX9 in the presence of 5 mM ATP or in the presence 5 mM AMP-PNP, a nonhydrolyzable nucleotide analogue. Triangle; as specified in Figure 1.