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. Author manuscript; available in PMC: 2011 Aug 24.
Published in final edited form as: Biochemistry. 2010 Aug 24;49(33):6992–6999. doi: 10.1021/bi100795m

Figure 5. DHX9 activity requires a 3′-single-stranded overhang and displays 3′→5′ polarity.

Figure 5

Panel A. Lanes 1–2, mobility of the single-strands used to prepare the duplex and blunt-end triplex DNA structures. Helicase activity (see “Experimental Procedures”) in the presence of ATP was assessed on blunt-end duplex DNA (lanes 3–4) and blunt-end triplex DNA (lanes 5–8) by using 200 and 0–200 nM DHX9, respectively, whereas lane 9 shows the helicase activity of 200 nM DHX9 on blunt-end triplex in the absence of ATP. Triangle; as specified in Figure 1. Panel B. Helicase activity of DHX9 on various types of triplex structures. Lanes 1–3, mobility of the single-strands used to assemble triplex DNA structures with blunt-ends (lanes 5–6), 5′-single-stranded overhang (lanes 7–8) and 3′-single-stranded overhang (lanes 9–10) as depicted above the lanes, lane 4 shows the mobility of duplex DNA. A total of 200 nM DHX9 was used to determine the helicase activity on each type of triplex DNA structure.