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. Author manuscript; available in PMC: 2010 Oct 5.
Published in final edited form as: Methods Mol Biol. 2009;582:233–244. doi: 10.1007/978-1-60761-340-4_18

Fig. 4.

Fig. 4

Recovery of EGFP–TopoIIα fluorescence in interphase nuclei using pattern photobleaching with the Mosaic argon laser system. (A) Control cell nucleus shows rapid recovery of fluorescence after photobleaching (B) The topoisomerase II inhibitor ICRF159 causes a change in TopoIIα distribution in interphase nuclei resulting in a more granular appearance and a relative decrease in concentration of TopoIIα in the nucleolus. ICRF159 greatly slows recovery of photobleaching in the nucleus although recovery is somewhat more rapid within the nucleolus even in the presence of the drug. Images include a prebleach image, one taken just after photobleaching (0 time) and images taken during recovery. Time is depicted as minutes:seconds after photobleaching. Bar = 10 µm.