FIG. 4.

Synthetic triterpenoid, CDDO-Me pretreatment preserves levels of innate and adaptive immune cell populations in spleen, while lipopolysaccharide (LPS) pretreatment reduces splenocyte immune cell populations. (A) Innate immune cell populations. Spleens were harvested from individual mice in each group and pooled (n = 4 spleens per group) prior to being stained with conjugated monoclonal antibodies (MAb) and analyzed using flow cytometry. Data are absolute numbers (× 10⁶) of indicated innate immune cells measured from pooled splenocytes as described in the Materials and Methods. Total myeloid (CD11b⁺) cells were further classified as macrophages (CD11b⁺Gr-1^-) and polymorphonuclear cells (CD11b⁺Gr-1⁺) (Left figure). Total dendritic cells (CD11c⁺CD11b⁺) were further classified as myeloid (CD11c⁺CD8^-), lymphoid (CD11c⁺CD8⁺), and plasmacytoid (CD11c⁺B220⁺Ly6C⁺) (Right figure). (B) Adaptive immune cell populations. Absolute numbers (× 10⁶) of B cells (B220⁺) (Left figure) and T-cell subtypes (CD3⁺CD4⁺; CD3⁺CD8⁺; and CD25 expression on gated CD3⁺CD4⁺cells, CD4⁺CD25⁺) (Right figure) are shown. Representative results are from 1 of 3 independent experiments.