FIG. 5.

Splenocyte CD4⁺IL-17⁺ (Th17) cells are increased following lipopolysaccharide (LPS) pretreatment, but are not increased following synthetic triterpenoid, CDDO-Me pretreatment. Whole spleens from individual mice (n = 1 spleen per indicated pretreatment group) were used to define splenocyte Th17 cell content. Indicated percentages are percentages of total cells as determined by analytical software. Relevant isotype controls were used to set the illustrated region gates (data not shown). Axes to histograms for each row are indicated to the left of the histograms. Representative results are from 1 of 3 independent experiments.