FIG. 7.

Levels of TLR-inducible IL-6 (A) and IL-10 (B) differ between lipopolysaccharide (LPS)-pretreated mice and mice pretreated with synthetic triterpenoid, CDDO-Me. Levels in inducible IL-6 and IL-10 (mean ± SD) were measured from supernatants of splenocyte cultures from control (CL), LPS (LPS)-, vehicle (DMSO)-, and CDDO-Me pretreated mice. Each stimulation condition was performed in triplicate. Unselected splenocytes (1 × 10⁷ cells/mL) were stimulated with media alone (TCM/1%NDM) or media containing R848 (Resiquimod) (1 μg/ml) or CpG ODN 1826 (10 μg/ml). Twenty-four hour supernatants were collected for ELISA. Mean inducible levels of IL-6 and IL-10 among experimental groups and across stimulation conditions were significantly different (P < 0.0001, 2-way ANOVA).