Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jul;30(7):513–523. doi: 10.1089/jir.2009.0105

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright 2010, Mary Ann Liebert, Inc.

PMC Copyright notice

FIG. 3. — Unilateral ureteral obstruction (UUO) induces the expression of Oncostatin M (OSM) and OSM receptor mRNA. (A) Time course of OSM mRNA expression in left obstructed rat kidneys, and control kidneys (control) 1, 3, 6, 12, and 24 h after UUO. Ribonuclease protection assays (RPA) were performed with total RNA prepared from an entire kidney to quantitate mRNA levels for OSM and control ribosomal protein L32 (rPL32). Representative gel segments from 2 (a, b) obstructed and 2 control kidneys per time point showed a progressive up-regulation of mRNA encoding for OSM during the course of UUO as compared with control kidney. The sizes of protected fragments for both mRNA species are indicated with arrows. (Below) Semiquantitative data using densitometric analysis of OSM relative to L32. *P < 0.05. (B) Renal expression of OSM and OSM receptor mRNA was determined by real-time RT-PCR from total renal isolates at designated time points after unilateral ureteral obstruction (UUO) in the mouse or rat. Data are presented as the fold increase of OSM mRNA expression in obstructed kidneys (UUO) normalized to non-ligated control kidneys (time 0). Each bar represents the mean ± SEM for a group of 3 animals. *P < 0.01, **P < 0.05 versus normal controls.

FIG. 3. — Unilateral ureteral obstruction (UUO) induces the expression of Oncostatin M (OSM) and OSM receptor mRNA. (A) Time course of OSM mRNA expression in left obstructed rat kidneys, and control kidneys (control) 1, 3, 6, 12, and 24 h after UUO. Ribonuclease protection assays (RPA) were performed with total RNA prepared from an entire kidney to quantitate mRNA levels for OSM and control ribosomal protein L32 (rPL32). Representative gel segments from 2 (a, b) obstructed and 2 control kidneys per time point showed a progressive up-regulation of mRNA encoding for OSM during the course of UUO as compared with control kidney. The sizes of protected fragments for both mRNA species are indicated with arrows. (Below) Semiquantitative data using densitometric analysis of OSM relative to L32. *P < 0.05. (B) Renal expression of OSM and OSM receptor mRNA was determined by real-time RT-PCR from total renal isolates at designated time points after unilateral ureteral obstruction (UUO) in the mouse or rat. Data are presented as the fold increase of OSM mRNA expression in obstructed kidneys (UUO) normalized to non-ligated control kidneys (time 0). Each bar represents the mean ± SEM for a group of 3 animals. *P < 0.01, **P < 0.05 versus normal controls.