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. Author manuscript; available in PMC: 2011 Sep 21.
Published in final edited form as: Sci Signal. 2010 Sep 21;3(140):ra67. doi: 10.1126/scisignal.2001083

Table 2.

Genes that when knocked down sensitized multiple cell lines to drugs. Threshold analysis provides a set of target genes based on strict statistical criteria. Rank order analysis provides a different view of the target gene set by compensating for stochastic noise in the responsiveness of cell lines (including DLD-1, DKS-8, LoVO, MIA PaCa-2, Panc1, and HCT116) with nonspecific drug-resistance mutations, such as in the genes encoding K-RAS or p53. For rank analysis, genes indicated represent those found in the highest and lowest quartiles based on absolute activity for each of the categories specified.

Cutoff Analysis Only Rank Analysis Only
Genes that sensitize A431 cells to erlotinib and cetuximab ASCL2
CDH3
CXCL12
DIXDC1
DLG4
DUSP7
FLNA
INPPL1
PLSCR1
PPIAP19
PRKACB
PRKCE
PRKCZ
RPS6KA5
SC4MOL
SH2D3C
CDH3
CXCL12
FLNA
INPPL1
PRKACB
PRKCE
RPS6KA5
SC4MOL
SH2D3C
Genes that sensitize two or more cell lines to erlotinib AKT2
ASCL2
BCAR1
CD59
DIXDC1
DUSP7
FLNA
LTK
PIK3R2
PRKACB
PRKCD
PRKCE
RPS6KA5
SC4MOL
SH2D3C
AKT2
ASCL2
BCAR1
CD59
DIXDC1
DLG4
DUSP7
FLNA
KLF10
LTK
NEDD9
PRKACB
PRKCE
SC4MOL
SH2D3C
SHC1
Genes that sensitize one or more resistant cell lines to erlotinib AKT2
BCAR1
CD59
DIXDC1
DUSP7
EPHA5
NEDD9
PIK3R2
PRKACB
SC4MOL
SH2D3C
SHC1
AKT2
ANXA6
ASCL2
BCAR1
CD59
DIXDC1
DLG4
DUSP6
DUSP7
EPHA5
FGFR2
KLF10
LOC63920
LTK
NEDD9
PKN2
PPIAP19
PRKACB
RET
SC4MOL
SH2D3C
SHC1
Genes that sensitized cells to both erlotinib and CPT11 in >3 cell lines, none BCAR1
CD59
DLG4
Genes that sensitized cells to erlotinib and CPT11, but did not reduce viability in the absence of drug CXCL12 (only in A431) DLG4
DUSP6
Genes that affected both the viability and sensitization to erlotinib or CPT11 in ≥ 2 tumor cells lines ASCL2
BCAR1
DUSP7
ERBB3
FLNA
INPPL1
LTK
PIK3R2
PRKACB
PRKCD
PRKCE
RASA3
RPS6KA5
SC4MOL
SH2D3C
SHC1
SOS2
VAV3
DIXDC1
FLNA
PRKCE
SH2D3C
SHC1
Genes that reduced viability of at least 2 RAS mutant cell lines but not more than one wild-type cell line AKT2
ARF4
CDH3
CXCL12
DUSP7
FLNA
LOC63920
PPIAP19
ANXA6
FLNA
PPIAP19
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