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Roscovitine inhibits replication of the paternal centrosome. Sea urchin zygotes were treated with 20 µM roscovitine at 1.5 minutes or 15 minutes post-fertilization. Replication of the paternal centrosome was assayed for using α-tubulin immunofluorescence. Only one aster was observed in zygotes treated at 1.5 minutes post-fertilization (A), whereas zygotes treated at 15 minutes contained two asters (B).
