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Alterations in protein expression of calpain, SBDP, calpastatin, cathepsin D, and APP. Cells were treated with various doses of IFN-γ (100, 300, and 500 units/ml) for 48 hours. (A) Representative Western blots to show levels of 80 kD calpain, 145 kD SBDP, 110 kD calpastatin, 46 kD and 33 kD cathepsin D, 100 kD APP, and 37 kD GAPDH. (B) Determination of changes in calpain:calpastatin and SBDP: calpastatin ratios. (C) Determination of percent changes in 33 kD cathepsin D and 100 kD APP.
