Figure 3.

Repeated ethanol administration results in an increase in contribution of NR2B subunits to the activity of the channel in the DMS. A, Time course of NMDAR-EPSCs in DMS neurons from ethanol- and saline-treated rats before and during bath application of the NR2B-NMDAR-specific antagonist, Ro 25-6981 (0.25 μm) (as indicated by the horizontal line). Rats received injections of ethanol (2 g/kg, i.p.) or saline once a day for 7 d. DMS slices were prepared 16 h after the last injection. n = 17 (saline) and 15 (EtOH) slices. B, Summary of the mean inhibition magnitude of NMDAR-EPSCs by Ro 25-6981 in DMS neurons from saline- and ethanol-treated animals. *p < 0.05, t test.