Figure 5.

Repeated administration of ethanol increases Fyn activation in the DMS. Rats were treated with ethanol (2 g/kg, i.p.) or saline once a day for 7 d. DMS tissue was dissected out 16 h after the last treatment. Fyn kinase in total homogenates was immunoprecipitated with anti-Fyn antibodies (A, left panel) and anti-mouse IgG antibodies as a control (A, right panel). Active Fyn kinase was detected by the anti-pY420/418[Fyn/Src] antibodies. The blot was stripped and reprobed first with antibodies that recognize the inactive form of the kinase (anti-pY531/529[Fyn/Src] antibodies), and second with anti-Fyn antibodies (to measure the level of immunoprecipitated Fyn). Images are representative of n = 12 for each treatment. B, Bar graph summarizing the averaged effect of ethanol treatment on the levels of the phosphorylation state of Fyn by quantification of the level of pY420/418 or pY531/529 to total immunoprecipitated Fyn. *p < 0.05 versus saline (Mann–Whitney rank sum test).