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. 2010 Jul 28;30(30):10187–10198. doi: 10.1523/JNEUROSCI.2268-10.2010

Figure 7.

Figure 7.

Intermittent access to ethanol results in increased phosphorylation of NR2B subunits in the DMS. Rats were exposed to 18 drinking sessions using the intermittent access to 20% ethanol two-bottle choice procedure. One day after the last ethanol-drinking session, DMS tissue was dissected out and protein and phosphorylation levels of NR2B subunits in homogenates were measured. A, Intermittent access to ethanol results in increased phosphorylation levels of NR2B subunits in the DMS. Phospho-NR2B levels were measured using anti-[pY1472]NR2B (p-NR2B) antibodies (top). Bar graphs summarizing the averaged changes in phosphorylation levels of NR2B subunits (bottom). Western blot data were normalized to GAPDH and plotted as percentage of water control. *p < 0.05 versus water (two-tailed t test). n = 7 (water). n = 8 (EtOH). B, Intermittent access to ethanol does not alter the protein levels of NR2B subunits in total DMS homogenates. Top, Image is representative of n = 7 (water) and 8 (EtOH). Bottom, Bar graphs summarizing the averaged changes in protein levels of NR2B subunits in total homogenates. p > 0.05 EtOH versus water (two-tailed t test).