Figure 3. Immunostaining of myotube cultures with the muscle-specific marker desmin.

Myotubes at 7 days post-differentiation were immunostained with desmin antibody and then cell nuclei were stained with Hoescht. Representative immunofluorescence micrographs of skeletal muscle cultures are shown: (A,B,E,F) C1, (C,D) P1 and (G,H) P2. In A, C, E and G white bars represent 100 µm. In B, D, F and H white bars represent 50 µm.