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. 2010 Oct 5;5(10):e13165. doi: 10.1371/journal.pone.0013165

Figure 1. Identification of YopM interacting proteins by tandem-affinity-purification.

Figure 1

(A) HEK293T cells were infected with the indicated strains, lysed and extracts were subjected to western blotting with anti-YopM antibody. (B) J774A.1 cells were infected with deltaYopM(pYopM-CBP-SBP) and lysates were prepared 90 minutes after infection. After tandem affinity purification the beads were boiled and subjected to SDS-PAGE. Visible bands after Coomassie-staining were excised and subsequently analyzed by mass spectrometry.