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. 2010 Oct 5;5(10):e13165. doi: 10.1371/journal.pone.0013165

Figure 6. YopM delivered during infection induces protracted activation of endogenous RSK in J774A.1 cells.

Figure 6

J774A.1 cell were infected with WA-C(pTTSS) (left upper panel) or with WA-C(pTTSS+pYopM) (right upper panel) or with WA-deltaYopM (left lower panel) or with WA-deltaYopM(pYopM) (right lower panel) and harvested at the indicated time points. Bacteria were killed by the addition of gentamicin after 90 minutes. Cellular lysates were then subjected to western blotting and probed with the indicated antibodies.