Table 2.
The List of Primers Used in the Study is Displayed
| Primer | Sequence | bp | Temperature(°C) | Cycles | Source |
|---|---|---|---|---|---|
| Biglycan | F -GAT GGC CTG AAG CTC AA R -GGT TGT TGA AGA GGC TG |
406 | 55 | 30 | AF159382 |
| Collagen I | F -GAT GCG TTC CAG TTC GAG TA R -GGT CTT CCG GTG GTC TTG TA |
312 | 55 | 30 | Le Graverand et al. (1999)10 |
| Collagen II | F -GCA CCC ATG GAC ATT GGA GGG R -GAC ACG GAG TAG CAC CAT CG |
366 | 65 | 35 | S83370 |
| Collagen III | F -TTA TAA ACC AAC CTC TTC CT R -TAT TAT AGC ACC ATT GAG AC |
255 | 55 | 30 | S83371 |
| Decorin | F -TGT GGA CAA TGG TTC TCT GG R -CCA CAT TGC AGT TAG GTT CC |
419 | 60 | 31 | S76584 |
| GAPDH | F -TCA CCA TCT TCC AGG AGC GA R -CAC AAT GCC GAA GTG GTC GT |
293 | 55 | 25 | L23961 |
| Lumican | F -CTG CAG TGG CTC ATT CTA R -GAC CTC CAG GTA ATA GTT |
576 | 55 | 35 | Majima et al. (2000)11 |
| MMP-1 | F -TCA GTT CGT CCT CAC TCC AG R -TTG GTC CAC CTG TCA TCT TC |
649 | 55 | 32 | M17821 |
| MMP-13 | F -TTC GGC TTA GAG GTG ACA GG R -ACT CTT GCC GGT GTA GGT GT |
527 | 65 | 40 | AF059201 |
| TIMP-1 | F -GCA ACT CCG ACC TTG TCA TC R -AGC GTA GGT CTT GGT GAA GC |
326 | 60 | 32 | AF 156029 |
| TIMP-2 | F -CTC TGG AAA CGA CAT TTA TGG R -AGA TGT AGC ACG GGA TCA TGG |
416 | 60 | 29 | AF 156030 |
| TIMP-3 | F -GTG CAA CTT CGT GGA GAG R -GGT CTG TGG CAT TGA TGA |
454 | 60 | 29 | AF 156031 |
F = Forward; R = Reverse; bp = length of primer product in base pairs; temperature = annealing temperature; source = Genbank accession number or journal article reference; GAPDH = glyceraldehydes 3-phosphate dehydrogenase; MMP = matrix metalloproteinase; TIMP = tissue inhibitors of matrix metalloproteinase.