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. Author manuscript; available in PMC: 2010 Oct 5.
Published in final edited form as: J Immunol. 2009 Jul 1;183(1):261–269. doi: 10.4049/jimmunol.0803853

Figure 4.

Figure 4

MEK/ERK-mediated inhibition of Bim is critical for the survival but not the cycling of mitogenically stimulated T cells. Graphs show mean % apoptosis of all cells (A) or mean % cells in the S phase as a proportion of total live cells (B) of purified T cells from wt (black bars) or Bim−/− mice (white bars) at 0, 24 or 48 h after stimulation with anti-CD3 plus anti-CD28 mAbs plus IL-2, plus or minus the addition of the MEK1/2 inhibitor UO126 (10 µM). Data represent mean +/−SD of 3 independent mice of each genotype from 3 independent experiments. (C) Representative examples of cell cycle analyses by FACS analysis are shown for purified T cells from wt or Bim−/− T cells 48 h after stimulation with anti-CD3 plus anti-CD28 mAbs plus IL-2, plus or minus the addition of the MEK1/2 inhibitor UO126 (10 µM) or DMSO (diluent control).