Figure 7.
Bcl-2 over-expression completely prevents MEK/ERK inhibition induced apoptosis in mitogen stimulated B cells. The graph shows mean % apoptosis of purified B cells as a proportion of all B cells from wt (black bars) or vav-bcl-2 transgenic mice (white bars) at 0, 24 or 48 h after stimulation with Fab2 anti-IgM antibody fragments plus IL-2, IL-4, IL-5, plus or minus the addition of the MEK1/2 inhibitor UO126 (10 µM). Data represent mean +/−SD of cells from 3 mice of each genotype analyzed in 3 independent experiments. * P<0.05, t-test.