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. Author manuscript; available in PMC: 2010 Oct 5.
Published in final edited form as: Mol Cell Biochem. 2008 Jun 10;315(1-2):185–193. doi: 10.1007/s11010-008-9806-5

Fig. 1.

Fig. 1

Detection of Ang II type 1 (AT1) and type 2 (AT2) receptors in RIE-1 cell line. A, Total RNA was isolated from confluent RIE-1 cells cultured in serum-free medium as described in the Materials and Methods section. The expression of specific mRNA was detected by RT-PCR. B, Receptor protein expression was determined by ligand receptor–binding assay as described in the Materials and Method section using cultured whole intact cells and [125I](Sar1,Ile8)-Ang II. Data are representative of triplicate determinations.