Fig. 2.

Effect of Ang II on COX expression in RIE-1 cells. A. Concentration dependent expression of COX-2 protein in RIE-1 cells by Ang II. B. Time dependent expression of COX-2 mRNA in RIE-1 cells by Ang II (10⁻⁹ M). C. Time dependent expression of COX-2 and COX-1 protein by Ang II (10⁻⁸ M). The whole homogenates were subjected to SDS-polyacrylamide gel electrophoresis followed by Western blotting with anti-murine COX-1 and COX-2 antibodies. D. Quantification of COX-2 protein in panel C was carried out by scanning densitometry and averages of the expression levels (n=5) normalized by actin levels are displayed in the histogram. *, P≤0.05 as compared to the level of time 0h.