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Expression levels of Ang II receptors in the RIE-1 cells transfected with AT2 cDNA were assessed by ligand receptor-binding assay as described in the Materials and Methods section. AT2 protein expression was markedly increased by AT2 cDNA transfection, whereas AT1 expression was not significantly altered as compared to LacZ transfected cells. The averages of three separate experiments are displayed in the histogram.*, P≤0.05 as compared to the level of LacZ transfected cells.
