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. Author manuscript; available in PMC: 2010 Oct 5.
Published in final edited form as: Mol Cell Biochem. 2008 Jun 10;315(1-2):185–193. doi: 10.1007/s11010-008-9806-5

Fig. 6.

Fig. 6

Effect of Ang II on LPS-induced COX-2 expression in AT2 over-expressed RIE-1 cells. AT2 transfected RIE-1 cells were pretreated with LPS (1–5 µg/ml) and then stimulated with either Ang II or CGP-42112A (CGP, 10−10 or 10−8 M) for 8 h as indicated in the figure. Top panel, COX-2 protein expression was assessed by Western blot analysis as described in Materials and Methods. Bottom panel, Semi-quantification of COX-2 protein expression was carried out according to the method described in the Materials and Methods using scanning densitometry. The average expression levels normalized by actin level (n=3) are displayed in the histogram. *, P≤0.05 as compared to the level of LPS alone treatments.