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. Author manuscript; available in PMC: 2010 Oct 5.
Published in final edited form as: Traffic. 2008 Sep 18;9(12):2063–2072. doi: 10.1111/j.1600-0854.2008.00822.x

Figure 2.

Figure 2

Nuclear β-DG is full length. A) Cell fractionation of MEpG-C7 cells expressing the empty vector (vec) or wtDG complementary DNA (DG) and MEpL, MEpG, MEpE and EpH4 mammary epithelial cells. The same membrane was incubated with anti-α-DG, β-DG, β1 integrin, lamin B1 and calreticulin antibodies. The markers for nuclear lamin B1, plasma membrane β1 integrins and ER protein calreticulin demonstrate that the cell fractionations were clean. B) Representation of DG glycosylation sites and β-DG immunoblot of cell fractions treated with PNGase F or Endo H. C) Time-lapse imaging shows rapid recovery of fluorescence in nuclei of DG–eGFP-expressing cells in seconds after photobleaching. Bar 10 μm.