DG contains a functional NLS. A) Schematic representation of cyto-DG–eGFP constructs. The transmembrane domain is shaded. The mutants described are nls1: KKRK777NPGE and nls2: KK793TA, K823A, PP828AA and Y831A. B) Confocal images of MEpG-C7 cells infected with cyto-DG–eGFP or indicated mutants. Arrows point to representative cells shown in the insets. Bar 10 μm. C) Cell fractionation of MEpG-C7 cells expressing the empty viral vector, wtDG, nls1, nls2 or nls1 + 2 mutants. The same membrane was incubated with α-DG, β-DG, β1 integrin, lamin B1 and calreticulin antibodies.