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. Author manuscript; available in PMC: 2010 Oct 5.
Published in final edited form as: Traffic. 2008 Sep 18;9(12):2063–2072. doi: 10.1111/j.1600-0854.2008.00822.x

Figure 3.

Figure 3

DG contains a functional NLS. A) Schematic representation of cyto-DG–eGFP constructs. The transmembrane domain is shaded. The mutants described are nls1: KKRK777NPGE and nls2: KK793TA, K823A, PP828AA and Y831A. B) Confocal images of MEpG-C7 cells infected with cyto-DG–eGFP or indicated mutants. Arrows point to representative cells shown in the insets. Bar 10 μm. C) Cell fractionation of MEpG-C7 cells expressing the empty viral vector, wtDG, nls1, nls2 or nls1 + 2 mutants. The same membrane was incubated with α-DG, β-DG, β1 integrin, lamin B1 and calreticulin antibodies.