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SEA autoproteolysis and nuclear translocation of DG and MUC1. A) MUC1 western blot of BT549 cell fractions treated with PNGase F or Endo H. B) Fractions of MEpG-C7 cells expressing the empty viral vector (vec), wtDG or S654A mutant. The same membrane was probed with IIH6, β1 integrin and lamin B1 antibodies. C) Cell fractionation of BT549 cells expressing vec, wtMUC1 or S1098A mutant. The same membrane was probed with MUC1-C, β1 integrin and lamin B1 antibodies.
