Skip to main content
. Author manuscript; available in PMC: 2010 Oct 6.
Published in final edited form as: Aust N Z J Psychiatry. 2010 Jan;44(1):59–70. doi: 10.3109/00048670903393662

Figure 2.

Figure 2

Average expression stability and optimal number of housekeeper genes for normalisation determined using the program geNorm. (A) Genes were ranked according to average expression stability. ACTB and GAPDH were the most stable genes (lowest M value), while GUSB was the least stable gene (highest M value). (B) Pairwise variation with the stepwise addition of more housekeeping genes shows that the use of the two most stable housekeeping genes will be sufficient for accurate normalisation as this satisfies the cutoff (0.15M) recommended by Vandesompele et al (2002).