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. 2010 Aug 30;43(5):457–463. doi: 10.1111/j.1365-2184.2010.00695.x

Figure 3.

Figure 3

 Id1 induces proliferation of keratinocytes via NF‐κB in vitro. (A) Id1 significantly increased DNA synthesis (CPM, counts per minute) compared with vec, whereas PDTC eliminated the effect of Id on DNA synthesis of keratinocytes. (B) Id1 increased cell population growth rate in a time‐dependent manner. At 24 h, Id1 significantly increased growth rate compared to vec. PDTC significantly inhibited the effect of Id1 on growth rate. Note that empty vector‐transfected cells had high growth rate at 5 h but declined thereafter, whereas Id1‐transfected cells had low growth rate at 5 h but increased thereafter. (C) Id1 significantly increased promoter activity of NF‐κB (RLA, relative luciferase activity) compared to vec and PDTC eliminated the effect of Id1 on promoter activity of NF‐κB. (D) Id1 increased NF‐κB translocation into nuclei of Rhek‐1A keratinocytes after 1 h transfection of Id1 compared to vec (arrows in panel b pointing to the nuclei of Rhek‐1A keratinocytes, positive for activated NF‐κB after staining with anti‐activated NF‐κB). Bar = 10 μm, applying to the same row in (D). med, medium alone as blank control; vec, empty vector as transfection control; *P < 0.05.